Anti-ABHD7 Antibody

EPHX4 antibody

Boster Bio Anti-ABHD7 Antibody catalog # A16314. Tested in WB applications. This antibody reacts with Human, Mouse.

Product Info Summary

SKU: A16314
Size: 100μl
Reactive Species: Human, Mouse
Host: Rabbit
Application: WB

Product Name

Anti-ABHD7 Antibody

View all EPHX4 Antibodies

SKU/Catalog Number

A16314

Size

100μl

Form

Liquid

Description

Boster Bio Anti-ABHD7 Antibody catalog # A16314. Tested in WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ABHD7 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A16314)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

1B9C1

Isotype

IgG

Immunogen

Synthesized peptide derived from the Internal region of human ABHD7. at AA rangle: 70-150

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A16314 is reactive to EPHX4 in Human, Mouse

Applications

A16314 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

42.324kDa

Background of EPHX4

BIVM (for basic, immunoglobulin-like variable motif-containing) refers to a recently identified gene product that maps to human chromosome 13q32-q33 and is predicted to encode a 503-amino acid protein. BIVM shows ubiquitous expression in normal human tissue and the presence of a 5' CpG island suggests it is a housekeeping gene. BIVM is likely essential for some aspect of basic cellular function. BIVM is highly charged and localizes to the cytoplasm and nucleus where it may bind to either DNA or RNA or associate with other cellular proteins. Significant sequence homology exists with many organisms.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For EPHX4 (Source: Uniprot.org, NCBI)

Gene Name

EPHX4

Full Name

Epoxide hydrolase 4

Weight

42.324kDa

Superfamily

AB hydrolase superfamily

Alternative Names

ABHD7; abhydrolase domain containing 7; Abhydrolase domain-containing protein 7; EC 3.3; EPHXRPEpoxide hydrolase-related protein; epoxide hydrolase 4; FLJ90341 EPHX4 ABHD7, EH4, EPHXRP epoxide hydrolase 4 epoxide hydrolase 4|abhydrolase domain containing 7|abhydrolase domain-containing protein 7|epoxide hydrolase-related protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on EPHX4, check out the EPHX4 Infographic

EPHX4 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for EPHX4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-ABHD7 Antibody

Question

Does A16314 anti-ABHD7 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-01-14

Answer

It shows on the product datasheet, A16314 anti-ABHD7 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-01-14

Question

Is this A16314 anti-ABHD7 antibody reactive to the isotypes of EPHX4?

Verified Customer

Verified customer

Asked: 2019-12-25

Answer

The immunogen of A16314 anti-ABHD7 antibody is Synthesized peptide derived from the Internal region of human ABHD7. at AA rangle: 70-150. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-12-25

Question

We are currently using anti-ABHD7 antibody A16314 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2019-08-27

Answer

The anti-ABHD7 antibody (A16314) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-08-27

Question

Can you help my question with product A16314, anti-ABHD7 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-05-28

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A16314 anti-ABHD7 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-05-28

Question

See attached the WB image, lot number and protocol we used for caudate nucleus using anti-ABHD7 antibody A16314. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2018-05-02

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-05-02

Order DetailsPrice
A16314

100uL

$399

Free Secondary Antibody

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A16314
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$399.00

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