Product Info Summary
SKU: | A16314 |
---|---|
Size: | 100μl |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-ABHD7 Antibody
SKU/Catalog Number
A16314
Size
100μl
Form
Liquid
Description
Boster Bio Anti-ABHD7 Antibody catalog # A16314. Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ABHD7 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A16314)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
1B9C1
Isotype
IgG
Immunogen
Synthesized peptide derived from the Internal region of human ABHD7. at AA rangle: 70-150
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A16314 is reactive to EPHX4 in Human, Mouse
Applications
A16314 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
42.324kDa
Background of EPHX4
BIVM (for basic, immunoglobulin-like variable motif-containing) refers to a recently identified gene product that maps to human chromosome 13q32-q33 and is predicted to encode a 503-amino acid protein. BIVM shows ubiquitous expression in normal human tissue and the presence of a 5' CpG island suggests it is a housekeeping gene. BIVM is likely essential for some aspect of basic cellular function. BIVM is highly charged and localizes to the cytoplasm and nucleus where it may bind to either DNA or RNA or associate with other cellular proteins. Significant sequence homology exists with many organisms.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
Click image to see more details
Western Blot (WB) analysis of specific cells using ABHD7 Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For EPHX4 (Source: Uniprot.org, NCBI)
Gene Name
EPHX4
Full Name
Epoxide hydrolase 4
Weight
42.324kDa
Superfamily
AB hydrolase superfamily
Alternative Names
ABHD7; abhydrolase domain containing 7; Abhydrolase domain-containing protein 7; EC 3.3; EPHXRPEpoxide hydrolase-related protein; epoxide hydrolase 4; FLJ90341 EPHX4 ABHD7, EH4, EPHXRP epoxide hydrolase 4 epoxide hydrolase 4|abhydrolase domain containing 7|abhydrolase domain-containing protein 7|epoxide hydrolase-related protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EPHX4, check out the EPHX4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EPHX4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ABHD7 Antibody (A16314)
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No publications found for A16314
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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5 Customer Q&As for Anti-ABHD7 Antibody
Question
Does A16314 anti-ABHD7 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-01-14
Answer
It shows on the product datasheet, A16314 anti-ABHD7 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-01-14
Question
Is this A16314 anti-ABHD7 antibody reactive to the isotypes of EPHX4?
Verified Customer
Verified customer
Asked: 2019-12-25
Answer
The immunogen of A16314 anti-ABHD7 antibody is Synthesized peptide derived from the Internal region of human ABHD7. at AA rangle: 70-150. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-12-25
Question
We are currently using anti-ABHD7 antibody A16314 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-27
Answer
The anti-ABHD7 antibody (A16314) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-27
Question
Can you help my question with product A16314, anti-ABHD7 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-05-28
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A16314 anti-ABHD7 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-28
Question
See attached the WB image, lot number and protocol we used for caudate nucleus using anti-ABHD7 antibody A16314. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-05-02
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-05-02